Culture of animal cells

a manual of basic technique and specialized applications
  • 0.88 MB
  • 3215 Downloads
  • English
by
John Wiley & Sons , Hoboken, N.J
Tissue culture -- Laboratory manuals, Cell culture -- Laboratory ma
StatementR. Ian Freshney.
GenreLaboratory manuals
Classifications
LC ClassificationsQH585.2 .F74 2010
The Physical Object
Paginationp. cm.
ID Numbers
Open LibraryOL24098939M
ISBN 139780470528129
LC Control Number2010007042
OCLC/WorldCa553361168

Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Sixth Edition. Ian Freshney. First published: 20 September Print ISBN: | Online ISBN: | DOI: / Author Biography.

About this book. This is the sixth edition of the leading text in the basic methodology of. Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition provides the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells.

This text is an indispensable resource for those in or entering the field, including academic research scientists Cited by: Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition provides the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells.

This text is an indispensable resource for those in or entering the field, including academic research scientists. A new type of perfusion reactor, based on a homogeneous reactor equipped with a microfiltration module on an external loop, yields significant increase in culture performances with OKT3 hybridoma: x10 7 cells/ml, mg MAb/day.L and g MAb/L of spent serum, compared to 2x10 6 cells/ml, 12 mg MAb/day.L and g MAb/L of spent serum in a.

Ian Freshney, PhD, is an honorary Senior Research Fellow in the Centre for Oncology and Applied Pharmacology at the University of Glasgow. He is a world-renowned expert on cell culture technique and has authored and edited numerous successful books, including the hugely popular Culture of Animal Cells (now in its 6th Edition), and Culture of Human Stem Cells/5(29).

is a platform for academics to share research papers. Ian Freshney has 12 books on Goodreads with ratings. Ian Freshney’s most popular book is Culture of Animal Cells: A Manual of Basic Technique.

Tissue culture involves the in vitro maintenance and propagation of cells in optimal conditions. Culturing animal cells, tissue or organs in a controlled artificial environment is called animal tissue culture.

The importance of animal tissue culture was initially realized during the development of the polio vaccine using primary monkey kidney cells (the polio vaccine was the first commercial.

Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition provides the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells.

This text is an indispensable resource for those in or entering the field, including academic research scientists 4/5. Cell culture technology is considered one of the major breakthroughs in the life sciences. This technology involves the removal of cells, tissues or organs from an animal or plant and their subsequent placement into an artificial environment conducive to their survival and/or proliferation [1–7].The optimal environmental conditions required for cell growth are a controlled temperature, a.

Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications (6th ed.) by R. Ian Freshney.

This is the sixth edition of the leading text in the basic methodology of. Culture of Animal Cells book. Read 2 reviews from the world's largest community for readers.

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The most complete resource on the techniques, equipment, pri 4/5. Animal cells are more difficult to culture than microorganisms because they require many more nutrients and typically grow only when attached to specially coated surfaces. Despite these difficulties, various types of animal cells, including both undifferentiated and differentiated ones, can be cultured by: 2.

Summary This chapter contains sections titled: The Culture Environment Cell Adhesion Cell Proliferation Differentiation Cell Signaling Energy Metabolism Origin of Cultured Cells.

Description of the book "Culture of Animal Cells: A Manual of Basic Technique": This is the most complete resource on the techniques, equipment, principles, and practices of animal cell culture. Since publication of the previous edition of this benchmark text, numerous groundbreaking advances have occurred in stem cell research, cloning, tissue.

Culture of Animal Cells by R. Ian Freshney,available at Book Depository with free delivery worldwide.3/5(2).

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one of the first major commercial products of cultured animal cells. Since the s a range of other products synthesized from animal cells have found commercial application. Consequently, the study of the optimization of culture conditions to maintain consistently high productivity from such animal cells in vitro has become of increased.

Book Description. The most complete resource on the techniques, equipment,principles, and practices of animal cell culture. Since publication of the previous edition of this benchmark text, numerous groundbreaking advances have occurred in stem cell research, cloning, tissue engineering, and in vitro toxicity testing.

Read culture of animal cells online, read in mobile or Kindle. Animal cell and tissue culture is an integral part of biotechnology and this book covers all the aspects of animal cell culture. Animal cells are used for making new vaccines, specific animal proteins such as intergerons, blood factors and hormones, monoclonal antibodies for use.

He is a world-renowned expert on cell culture technique and has authored and edited numerous successful books, including the hugely popular Culture of Animal Cells (now in its 6th Edition), and Culture of Human Stem Cells. Culture of Animal Cells: A Manual of Basic Technique and Specialized Application, 6th Ed has been very well received by the.

Animal Cell Bioreactors provides an introduction to the underlying principles and strategies in the in vitro cell culture biotechnology. It addresses engineering aspects such as mass transfer, instrumentation, and control ensuring successful design and operation of animal cell bioreactors.

Description Culture of animal cells FB2

Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition provides the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells.

This text is an indispensable resource for those in or entering the field, including academic research scientists /5(17). Introduction: The use of animal cell culture 1. Tissue culture, organ culture and cell culture Cells, removed from animal tissue or whole animals, will continue to grow if supplied with nutrients and growth factors.

This process is called cell culture. It occurs in vitro (‘in glass’) as opposed to. Contents. Culture of Animal Cells A Manual of Basic Technique and Specialized Applications, Sixth Edition Introduction 1 Training Programs 11 Biology of Cultures Cells 31 Laboratory design and Layout 43 Equipment 55 Aseptic Technique 73 Safety, Bioethics, and Validation 87 Culture Vessels and Substrates Defined Media and Supplements Serum-Free Media Preparation and Sterilization.

ADVERTISEMENTS: Read this article to learn about the fundamentals, facilities, aseptic conditions, advantages, disadvantages of animal cell culture and also about the risks & safety regulations in a tissue culture laboratory.

Introduction to Animal Cell Culture: Animal cell culture basically involves the in vitro (in the laboratory) maintenance and propagation of animal cells in [ ]. Comprised of chapters, this book begins with an overview of the problems and benefits of animal cell culture, followed by a discussion on the isolation of immortal murine macrophage cell lines.

The reader is systematically introduced to the use of DNA fingerprinting to characterize cell banks; immortalization of cells with oncogenes; lipid Book Edition: 1. Because of the continuing expansion of cell culture, and to keep the bulk of the book to a reasonable size, some specialized protocols are presented as supplementary material e of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition provides the most accessible and comprehensive introduction.

Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition provides the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells.

This text is an indispensable resource for those in or entering the field, including academic research scientists 2/5(1). Tool for the study of animal cell biology using convenient in vitro model of cell growth Mimic of in vivo cell behaviour (e.g cancer cells) Artificial (some cell types are thus difficult to culture) Highly selective and defined environment which.

Welcome to the companion site for the sixth edition of The Culture of Animal Cells by R. Ian Freshney. On this site you will find: Figures from the book as Powerpoints for downloading A list of COAC suppliers ; A database of cross contaminated or misidentified cell lines; Resources are displayed in two ways.

Cell culture refers to the removal of cells from an animal or plant and their subsequent growth in a favorable artificial environment. The cells may be removed from the tissue directly and disaggregated by enzymatic or mechanical means before cultivation, or they may be derived from a cell line or cell strain that has already been established.- Buy Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications book online at best prices in India on Read Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications book reviews & author details and more at Free delivery on qualified orders/5(28).Book reviews Freshney, R.I., Culture of Animal Cells: A Manual of Basic Technique, 3rd edn., pp., WileyLiss, New York, USDISBN This is a revised and expanded version of the widely known manual of techniques for culturing mammalian cells.